Ancient Duo teaches you to identify the authenticity of the ELISA kit!
Authentic identification method for ELISA kit:
1. Using the interference test to identify whether the ELISA kit detects the antigen of interest, as follows:
(1) The recombinant antibody antigen specific for the kit and the detection antibody in the kit are configured as a mixed incubation solution of antibody:antigenâ‰ˆ1:2
(2) After incubation at 37 Â° C for 15 minutes, replace the detection antibody in the original kit
(3) Follow-up operation according to the kit instructions, plus detection of antibodies, enzyme complexes and substrates
(4) After the experiment is completed, the standard curve is tested. If the standard curve is good, the added target antigen and the kit antibody do not match, and the kit detection antibody is for the non-target antigen, and the kit is a counterfeit ELISA kit.
(5) If the standard curve is wireless, it means that the detection antibody of the kit has been neutralized or interfered by the antigen of interest, which affects the detection of the actual kit antibody, and the kit detects that the antibody targets the antigen of interest.
2. If you have purchased two boxes of the same company's ELISA kits A and B, you can use the crossover experiment to identify whether the ELISA kit is fraudulent, as follows:
(1) Take out two of the coated plates of the purchased kit A.
(2) A standard curve of a coated plate plus kit A is used as a standard curve.
(3) Another standard of the coated plate plus kit B is used as a standard curve.
(4) Follow-up operation is carried out according to the instructions of the kit, and the detection antibody, enzyme complex and substrate are added.
(5) After the experiment is completed, test two standard curves, if two
The standard curve is consistent with the same indicator detected by the two ELISA kits instead of A and B, ie the kit is a counterfeit ELISA kit.
(6) If the two standard curves are inconsistent, the two indicators of the two ELISA kits are different. The reagent A can only detect A, and can not detect B, that is, the kit is a normal ELISA kit.
Company Name: Shanghai Guduo Biotechnology Co., Ltd.
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